Identification of Legionella Pneumophila Antigens and Antibodies by Immunoferritin Electronmicroscopy
نویسندگان
چکیده
منابع مشابه
Identification of protective B cell antigens of Legionella pneumophila.
Abs confer protection from secondary infection with Legionella pneumophila, the causative agent of a severe form of pneumonia known as Legionnaires' disease. In this study, we demonstrate that Ab-mediated protection is effective across L. pneumophila serogroups, suggesting that Abs specific for conserved protein Ags are sufficient to mediate this protective effect. We used two independent metho...
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Three monoclonal antibodies (McAb) were produced against soluble antigens of Legionella pneumophila serogroup 1 which was cultured on BCYE agar. The McAbs were all of the IgM isotype. The McAbs were used in the McAb-based ELISA for detection of circulating L. pneumophila antigens in 186 sera collected from patients with symptoms and signs suggestive of atypical pneumonia. The normal reference o...
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Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niches, African green monkey kidney epithelial (Vero) cells, A/J mouse bone marrow-...
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Legionella pneumophila remains a major health concern, especially for hospitalized patients. L. pneumophila in the environment can survive extracellular or as protozoan parasite within amoeba. After human infection it efficiently replicates in alveolar macrophages without activating inflammasome assembly and cleavage of caspase-1. In contrast murine macrophages actively recognize intracellular ...
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We read with interest the study by Diederen et al. (1), in which the serological assays from Vircell were evaluated. We found that this work underlines the importance of immunoglobulin M (IgM) detection in the diagnosis of legionellosis previously described by our group (3). Yet, we would like to point out some disagreements with some of the information given in the aforementioned study. The au...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 1982
ISSN: 0022-2615,1473-5644
DOI: 10.1099/00222615-15-2-181